Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Transformation of Xanthomonas axonopodis pv. citri by Electroporation
This study describes the use of electroporation for transforming Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus (Citrus spp.) canker. It also evaluates the methodology used for this species under different electrical parameters. The bacterium used in the study (Xac 306) was the same strain used for recent complete sequencing of the organism. The use of a plasmid (pUFR047, ge...
متن کاملXanthomonas axonopodis pv. allii
Scientific name: Xanthomonas axonopodis pv. allii (Roumagnac et al., 2004a,b). Synonyms: Xanthomonas campestris pv. allii (Kadota et al., 2000). Taxonomic position: Proteobacteria: Gammapropteobacteria: Xanthomonadales: Xanthomonasdaceae. Common names: Xanthomonas axonopodis pv. allii is the causal agent of onion bacterial blight (syn. Xanthomonas leaf blight of onion). EPPO code: XANTAA. Phyto...
متن کاملPlasmid Profile and Pulsed–Field Gel Electrophoresis Analysis of Salmonella enterica Isolates from Humans in Turkey
This study was conducted for typing Salmonella enterica subspecies enterica strains in Turkey using pulsed-field gel electrophoresis (PFGE) and plasmid DNA profile analysis. Fourty-two strains were isolated from clinical samples obtained from unrelated patients with acute diarrhea. The samples were collected from state hospitals and public health laboratories located at seven provinces in diffe...
متن کاملDifferential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the de...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics and Molecular Biology
سال: 2005
ISSN: 1415-4757
DOI: 10.1590/s1415-47572005000300020